7BHE
DARPin_D5/Her3 domain 4 complex, monoclinic crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.872, 62.254, 74.545 |
| Unit cell angles | 90.00, 102.41, 90.00 |
Refinement procedure
| Resolution | 44.400 - 2.297 |
| R-factor | 0.1873 |
| Rwork | 0.184 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y0b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4 (11-DEC-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.405 | 2.336 |
| High resolution limit [Å] | 2.296 | 2.296 |
| Rmerge | 0.131 | 0.515 |
| Rmeas | 0.147 | 0.576 |
| Rpim | 0.063 | 0.249 |
| Number of reflections | 24338 | 1250 |
| <I/σ(I)> | 5.4 | 2.1 |
| Completeness [%] | 93.2 | 97.4 |
| Redundancy | 4.52 | 4.44 |
| CC(1/2) | 0.993 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.83 | 293 | 0.1 M Na citrate, 12% PEG4000, 0.2 M Li sulfate |
