7BHA
Escherichia coli YtfE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-03-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7314 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.061, 50.647, 87.847 |
| Unit cell angles | 90.00, 100.54, 90.00 |
Refinement procedure
| Resolution | 59.050 - 2.190 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fnn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.464 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.050 | 2.188 |
| High resolution limit [Å] | 2.188 | 2.188 |
| Rmerge | 0.147 | 1.548 |
| Rmeas | 0.160 | |
| Rpim | 0.062 | |
| Number of reflections | 23568 | 549 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 87.0 | 40.2 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.985 | 0.354 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Tris-HCl, PEG 4000, magnesium chloride |
