7BH6
Room temperature, serial X-ray structure of CTX-M-15 collected on fixed target chips at Diamond Light Source I24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 294 |
| Detector technology | PIXEL |
| Collection date | 2019-05-09 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.98194 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.280, 45.958, 118.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.240 - 1.650 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.19720 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6qw8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.031 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.240 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 30552 | 1491 |
| <I/σ(I)> | 47.659 | 6.38 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 61.27 | 11.15 |
| CC(1/2) | 0.893 | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 2.0 M ammonium sulphate, 0.1 M Tris 8.0 |
