7BFD
Circular permutant of ribosomal protein S6, P54-55 truncated, Y4A mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.75142 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.041, 75.063, 223.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.570 |
| R-factor | 0.2587 |
| Rwork | 0.257 |
| R-free | 0.29720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.773 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.900 | 47.360 | 2.660 |
| High resolution limit [Å] | 2.570 | 9.950 | 2.570 |
| Rmerge | 0.174 | 0.044 | 1.310 |
| Rmeas | 0.189 | 0.048 | 1.454 |
| Rpim | 0.074 | 0.019 | 0.619 |
| Total number of observations | 3792 | 17082 | |
| Number of reflections | 33539 | 683 | 3160 |
| <I/σ(I)> | 8.5 | 30.6 | 1.1 |
| Completeness [%] | 99.6 | 99.1 | 97.6 |
| Redundancy | 6.5 | 5.6 | 5.4 |
| CC(1/2) | 0.997 | 0.999 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium citrate dibasic 20% w/v PEG 3350 |
