7BEX
Glyceraldehyde 3-phosphate dehydrogenase from Campylobacter jejeuni - ADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 90.250, 90.250, 223.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.105 - 2.054 |
| Rwork | 0.155 |
| R-free | 0.19050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7bew |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.709 |
| Data reduction software | iMOSFLM |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.670 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.130 | 0.320 |
| Number of reflections | 29280 | 2225 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 91.3 | 94.9 |
| Redundancy | 10.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 0.1M Sodium Acetate, 2.0 M Ammonium Sulfate, |
