7BEE
Crystal structure of a Hsp47-collagen peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-24 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.683, 96.683, 187.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.810 - 1.939 |
R-factor | 0.2087 |
Rwork | 0.207 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4au2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13rc2_2986) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.810 | 2.009 |
High resolution limit [Å] | 1.939 | 1.939 |
Rmerge | 0.050 | 0.863 |
Number of reflections | 75968 | 7346 |
<I/σ(I)> | 20.17 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 1.000 | 0.674 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES, 18-26% PEG 3350, 1-6% Tacsimate pH 7.0 |