7BE0
X-ray structure of Hen Egg White Lysozyme with dirhodium tetraacetate (2)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-13 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.948, 77.948, 37.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.990 - 1.620 |
| Rwork | 0.165 |
| R-free | 0.20280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.958 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.990 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.068 | 0.190 |
| Rpim | 0.021 | 0.152 |
| Number of reflections | 15018 | 623 |
| <I/σ(I)> | 56.7 | 3.4 |
| Completeness [%] | 98.7 | 86.6 |
| Redundancy | 9 | |
| CC(1/2) | 0.999 | 0.942 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M sodium acetate, 20% ethylene glicol, 0.6 M sodium nitrate |
