7BDN
Structure of the Streptomyces coelicolor small laccase - cubic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2019-04-24 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5419 |
Spacegroup name | P 21 3 |
Unit cell lengths | 176.981, 176.981, 176.981 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.500 - 2.700 |
R-factor | 0.2007 |
Rwork | 0.200 |
R-free | 0.22170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cg8 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.500 | 29.500 | 2.790 |
High resolution limit [Å] | 2.700 | 10.810 | 2.700 |
Rmerge | 0.166 | 0.048 | 0.742 |
Rmeas | 0.179 | 0.052 | 0.798 |
Rpim | 0.065 | 0.020 | 0.292 |
Total number of observations | 376482 | 5439 | 34207 |
Number of reflections | 50726 | 824 | 4638 |
<I/σ(I)> | 10.1 | 22.1 | 3 |
Completeness [%] | 99.9 | 95 | 99.6 |
Redundancy | 7.4 | 6.6 | 7.4 |
CC(1/2) | 0.995 | 0.999 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Protein concentration was 20 mg/ml in 20 mM Tris-HCl buffer (pH 7.5). Mother liquor was made up of 40% MPD, 20 mM NH4-OAc and 100 mM HEPES (pH 7.5). The protein was mixed in 2:1 ratio with protein to mother liquor. |