7BCX

The adduct of NAMI-A with Hen Egg White Lysozyme at 8 hours.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	78.211, 78.211, 37.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.070 - 1.060
R-factor	0.1622
Rwork	0.161
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.004
RMSD bond angle	0.751
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.041	1.098
High resolution limit [Å]	1.060	1.060
Rmerge	0.064
Number of reflections	53232	4334
<I/σ(I)>	26.55	2.26
Completeness [%]	98.3
Redundancy	24.5
CC(1/2)	0.998	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	NACL 1.5 M, 0.1 M NA ACETATE, PH 4.6, VAPOR DIFFUSION, SITTING DROP