7BCO

Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T in complex with D-foconate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-12
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.967
Spacegroup name	P 1 21 1
Unit cell lengths	58.166, 92.082, 62.378
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	31.250 - 2.000
R-factor	0.2032
Rwork	0.200
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bbr
RMSD bond length	0.014
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.250	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.030	0.290
Number of reflections	95881	9936
<I/σ(I)>	13.57	2.47
Completeness [%]	92.7
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	PEG4000