7BCB

Crystal structure of the HTH DNA binding protein ArdK from R388 plasmid bound to IR3 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	43.890, 77.220, 114.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.890 - 2.800
R-factor	0.2551
Rwork	0.253
R-free	0.29830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bca
RMSD bond length	0.006
RMSD bond angle	0.780
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (CCP4 7.0.078)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.220	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.084	0.362
Rmeas	0.102	0.446
Number of reflections	12277	1744
<I/σ(I)>	4.6	4.7
Completeness [%]	98.3
Redundancy	8.1
CC(1/2)	0.995	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	20% 10,000 polyethylene glycol, 0.1 M HEPES pH 7.5