7BBR
Crystal structure of the sugar acid binding protein DctPAm from Advenella mimigardefordensis strain DPN7T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.967 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.759, 61.850, 88.254 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.480 - 1.300 |
| R-factor | 0.1348 |
| Rwork | 0.133 |
| R-free | 0.16280 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.013 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.480 | 1.340 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.049 | |
| Rmeas | 0.054 | 0.320 |
| Number of reflections | 110532 | 10257 |
| <I/σ(I)> | 13.7 | 1.5 |
| Completeness [%] | 97.5 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 300 | 0.002 M zinc sulfate heptahydrate, 0.08 M HEPES pH 7.2 and 25 % Jeffamine ED 2003 |
