7BBN
Crystal structure of an ancient sequence-reconstructed Elongation Factor Tu (node 317)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.918401 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.342, 100.289, 160.719 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.440 - 1.680 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.22040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efc |
RMSD bond length | 0.013 |
RMSD bond angle | 1.851 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.23) |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.440 | 29.420 | 1.710 |
High resolution limit [Å] | 1.680 | 9.050 | 1.680 |
Rmerge | 0.093 | 0.062 | 0.834 |
Rmeas | 0.098 | 0.066 | 0.878 |
Rpim | 0.029 | 0.021 | 0.268 |
Total number of observations | 5821 | 37728 | |
Number of reflections | 88432 | 632 | 3787 |
<I/σ(I)> | 14.1 | 33.9 | 2.5 |
Completeness [%] | 98.9 | 97.2 | 83.2 |
Redundancy | 11 | 9.2 | 10 |
CC(1/2) | 0.999 | 0.997 | 0.785 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.2 M Lithium citrate tribasic tetrathydrate, 20% w/v PEG 3350, pH 8.4 |