7BB8
Crystal structure of Lugdulysin, a Staphylococcus lugdunensis M30 zinc metallopeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-28 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.590, 68.190, 122.960 |
| Unit cell angles | 90.00, 134.19, 90.00 |
Refinement procedure
| Resolution | 39.770 - 1.506 |
| Rwork | 0.156 |
| R-free | 0.17600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.770 | 1.560 |
| High resolution limit [Å] | 1.506 | 1.506 |
| Rmeas | 0.063 | |
| Number of reflections | 162434 | 14979 |
| <I/σ(I)> | 12.35 | |
| Completeness [%] | 95.8 | 62.73 |
| Redundancy | 5.4 | 1.4 |
| CC(1/2) | 0.999 | 0.264 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Sodium Cacodylate pH 6.5; 30% PEG 8000; 0.2 M Sodium acetate |
