7BAZ
Lysozyme crystallized in the presence of the hydrated deep eutectic solvent Choline chloride-Glycerol 1:2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.901, 78.901, 37.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.791 - 1.300 |
| R-factor | 0.1286 |
| Rwork | 0.127 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n9r |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | Sir2014 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.791 | 1.323 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.063 | 0.549 |
| Rmeas | 0.069 | 0.598 |
| Rpim | 0.026 | 0.234 |
| Number of reflections | 29376 | 1470 |
| <I/σ(I)> | 13.5 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.4 |
| CC(1/2) | 0.998 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | sodium chloride, sodium acetate, DES: Choline chloride/Glycerol |
