7B9R
Cys-45-tethered stabilizer 4 of 14-3-3(sigma)/ERa PPI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-04-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.071, 112.434, 62.496 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.035 - 1.150 |
R-factor | 0.1918 |
Rwork | 0.192 |
R-free | 0.19570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jc3 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3139) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 66.290 | 66.290 | 1.170 |
High resolution limit [Å] | 1.150 | 6.300 | 1.150 |
Rmerge | 0.050 | 0.017 | 1.550 |
Rmeas | 0.052 | 0.017 | 1.711 |
Rpim | 0.015 | 0.005 | 0.704 |
Total number of observations | 1199822 | 9074 | 21485 |
Number of reflections | 99856 | 708 | 3878 |
<I/σ(I)> | 24.8 | 126.7 | 1.1 |
Completeness [%] | 97.6 | 99.8 | 77.9 |
Redundancy | 12 | 12.8 | 5.5 |
CC(1/2) | 1.000 | 1.000 | 0.449 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 277 | 0.095 M HEPES, 0.19 M CaCl2, 27% (v/v) PEG400, 5% (v/v) glycerol |