7B9B

Crystal structure of human 5' exonuclease Appollo APO form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	90.417, 90.417, 104.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.210 - 2.800
R-factor	0.2683
Rwork	0.266
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5aho
RMSD bond length	0.003
RMSD bond angle	0.529
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.210	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.180	2.130
Rpim	0.040
Number of reflections	11188	1588
<I/σ(I)>	11.8	1.6
Completeness [%]	100.0	100
Redundancy	25.3	25.3
CC(1/2)	0.990	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	6% PEG20K -- 0.1M bicine pH 7.3