7B90
Circular permutant of ribosomal protein S6, P54-55 truncated, I8A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.331, 75.968, 222.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.000 - 2.050 |
| R-factor | 0.2632 |
| Rwork | 0.262 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.665 | 55.665 | 2.306 |
| High resolution limit [Å] | 2.050 | 6.855 | 2.082 |
| Rmerge | 0.169 | 0.054 | 0.909 |
| Rmeas | 0.185 | 0.061 | 0.990 |
| Rpim | 0.074 | 0.027 | 0.389 |
| Total number of observations | 10594 | 12782 | |
| Number of reflections | 39668 | 1982 | 1983 |
| <I/σ(I)> | 8 | 20.3 | 2 |
| Completeness [%] | 92.6 | 99.6 | 78.4 |
| Redundancy | 6.3 | 5.3 | 6.4 |
| CC(1/2) | 0.996 | 0.997 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium chloride 20% w/v PEG 3350 |
