7B8B

ADPG2 - ENDOPOLYGALACTURONASE FROM ARABIDOPSIS THALIANA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100.15
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	71.780, 88.560, 113.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.890 - 2.030
R-factor	0.1901
Rwork	0.188
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b7a
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	pointless
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	2.110
High resolution limit [Å]	2.030	2.030
Rmerge	0.021	0.323
Rmeas	0.029	0.456
Number of reflections	47350	4654
<I/σ(I)>	16.9
Completeness [%]	99.9	99.3
Redundancy	2	2
CC(1/2)	1.000	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Sodium malonate dibasic monohydrate, 20 % w/v PEG 3350 (E12 condition, PACT premier plate)