7B76
Crystal structure of the effector AvrLm5-9 from Leptosphaeria maculans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4878 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 57.900, 57.900, 212.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.810 - 2.700 |
| R-factor | 0.2781 |
| Rwork | 0.276 |
| R-free | 0.31160 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.749 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.810 | 2.860 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.299 | 2.220 |
| Number of reflections | 6425 | 997 |
| <I/σ(I)> | 8.75 | |
| Completeness [%] | 99.8 | |
| Redundancy | 11.2 | |
| CC(1/2) | 0.992 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.7M sodium acetate, 80 mM nickel sulfate, 0.1 M Hepes pH 7.5 |
