7B5V
The carbohydrate binding module family 48 (CBM48) and carboxy-terminal carbohydrate esterase family 1 (CE1) domains of the multidomain esterase DmCE1B from Dysgonomonas mossii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.962, 80.966, 107.350 |
| Unit cell angles | 90.00, 105.67, 90.00 |
Refinement procedure
| Resolution | 42.080 - 1.700 |
| R-factor | 0.1716 |
| Rwork | 0.168 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ne9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.875 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.080 | 1.757 |
| High resolution limit [Å] | 1.697 | 1.697 |
| Number of reflections | 94462 | 9133 |
| <I/σ(I)> | 9.34 | 0.89 |
| Completeness [%] | 99.6 | 96.87 |
| Redundancy | 6.8 | 6.7 |
| CC(1/2) | 0.998 | 0.588 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | JCSG - B9 - 0.1 M Citrate pH 5, 20% PEG 6000 |
