7B3A
Crystal structure of PamZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.941841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.966, 70.107, 54.552 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 20.200 - 1.340 |
| R-factor | 0.1479 |
| Rwork | 0.147 |
| R-free | 0.18060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g3s |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.499 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmeas | 0.068 | 129.200 |
| Number of reflections | 58898 | 9129 |
| <I/σ(I)> | 11.2 | 1 |
| Completeness [%] | 98.2 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.999 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 40% (w/v) PEG 3350, 50 mM ammonium sulfate, 100 mM sodium acetate at pH 4.6. |
