7B33
MST3 in complex with MRIA11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.972, 58.954, 61.441 |
| Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
| Resolution | 49.410 - 1.900 |
| R-factor | 0.1822 |
| Rwork | 0.181 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zhp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.839 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.045 | |
| Number of reflections | 27657 | 1776 |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 98.9 | 99.5 |
| Redundancy | 5 | 5.3 |
| CC(1/2) | 0.997 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein solution: 10 mg/ml in buffer 25 mM HEPES pH 7.5, 200 mM NaCl, 5% glycerol, 0.5 mM TCEP. Reservoir: 19% PEG 3350, 0.1M bis-tris pH 6.8. |
