7B32
MST3 in complex with MRIA7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.042, 59.212, 61.554 |
| Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
| Resolution | 49.900 - 1.750 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zhp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.785 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.024 | 0.209 |
| Number of reflections | 36097 | 1989 |
| <I/σ(I)> | 18.4 | 3.6 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 6.9 | 7.1 |
| CC(1/2) | 0.999 | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein solution: 10 mg/ml in buffer 25 mM HEPES pH 7.5, 200 mM NaCl, 5% glycerol, 0.5mM TCEP. Reservoir: 20% PEG 3350, 0.1M bis-tris pH 6.5. |
