7B2I

Heterodimeric tRNA-Guanine Transglycosylase from mouse

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.918400
Spacegroup name	P 43 2 2
Unit cell lengths	100.587, 100.587, 202.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.170 - 1.650
R-factor	0.1784
Rwork	0.177
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h62 6fv5
RMSD bond length	0.006
RMSD bond angle	0.773
Data reduction software	XDS (1.02)
Data scaling software	XDS (1.02)
Phasing software	PHASER (7.0.047)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.650	1.650
Number of reflections	125031	19850
<I/σ(I)>	23.17
Completeness [%]	99.9
Redundancy	13.2
CC(1/2)		0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	100 mM Citrate pH 6.5, 700 mM Ammonium sulfate, 1 M Lithium sulfate