7B2G

Crystal structure of R120Q GDAP1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9762
Spacegroup name	P 63 2 2
Unit cell lengths	148.111, 148.111, 114.538
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.430 - 3.000
R-factor	0.2329
Rwork	0.230
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7aia
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.430	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	15366	1410
<I/σ(I)>	18.31
Completeness [%]	99.9
Redundancy	38.5
CC(1/2)	0.999	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Succinic acid; 15% w/v PEG 3350