7B2A
Complement inhibitor CirpA5 from Rhipicephalus appendiculatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.010, 71.320, 110.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.080 - 1.910 |
| R-factor | 0.2207 |
| Rwork | 0.220 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CirpA1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.534 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.080 | 2.030 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 16268 | 1181 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.988 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium HEPES, pH 8.2, 50% (v/v) PEG500 MME |
