7B24
DtxR-like iron-dependent regulator IdeR (P39G variant) complexed with cobalt and its consensus DNA-binding sequence
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.395, 113.841, 89.795 |
| Unit cell angles | 90.00, 117.25, 90.00 |
Refinement procedure
| Resolution | 54.930 - 2.050 |
| R-factor | 0.2273 |
| Rwork | 0.226 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7b1v |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.930 | 2.230 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.082 | 1.305 |
| Rmeas | 0.088 | 1.411 |
| Rpim | 0.033 | 0.532 |
| Number of reflections | 76685 | 3835 |
| <I/σ(I)> | 12 | 1.7 |
| Completeness [%] | 93.1 | 61.6 |
| Redundancy | 7 | 6.9 |
| CC(1/2) | 0.998 | 0.573 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30%(w/v) PEG 2000 MME, 0.2 M ammonium sulfate, 0.1 M sodium acetate |
