7B22

Vibrio cholerae ParD2 Antitoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	C 2 2 21
Unit cell lengths	85.010, 101.700, 107.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.950 - 3.080
R-factor	0.2721
Rwork	0.271
R-free	0.29960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kxe
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.950	3.190
High resolution limit [Å]	3.080	3.080
Rmerge	0.174	1.410
Number of reflections	8845	842
<I/σ(I)>	7.36
Completeness [%]	99.5
Redundancy	6.8
CC(1/2)	0.996	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.2 M Lithium sulfate, 0.1 M MES pH 6 and 20 % w/v PEG 4000