7B1R
Crystal structure of B. subtilis glucose-1-phosphate uridylyltransferase YngB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 53.910, 158.610, 179.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.090 - 2.800 |
| R-factor | 0.1932 |
| Rwork | 0.191 |
| R-free | 0.24430 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.792 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.980 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.095 | 0.661 |
| Rpim | 0.067 | 0.468 |
| Number of reflections | 19457 | 2777 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M potassium citrate tribasic monohydrate, 0.05M lithium citrate tribasic tetrahydrate, 0.1M sodium phosphate monobasic monohydrate, 25% PEG6000 |
