7B0Q
In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus with H85A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.236, 45.140, 70.174 |
| Unit cell angles | 90.00, 121.38, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.420 |
| R-factor | 0.2197 |
| Rwork | 0.218 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7b0o |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.640 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmeas | 0.120 | 1.620 |
| Number of reflections | 8396 | 420 |
| <I/σ(I)> | 9.7 | 1.3 |
| Completeness [%] | 92.0 | 66.6 |
| Redundancy | 6.6 | 6.8 |
| CC(1/2) | 0.998 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.5 | 293 | 100 mM sodium citrate/HCl, pH 5.5, 75-150 mM NaCl, and 36-44 %(v/v) PEG 200 |
