7B0P

In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus in space group P21212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96860
Spacegroup name	P 21 21 2
Unit cell lengths	51.166, 192.235, 76.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 1.935
R-factor	0.2329
Rwork	0.232
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b0o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3494)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.280
High resolution limit [Å]	1.935	1.940
Rmeas	0.660	6.070
Number of reflections	29001	1451
<I/σ(I)>	4.4	1.7
Completeness [%]	89.5	47.4
Redundancy	5.4
CC(1/2)	0.730	0.162

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.5	293	100 mM sodium citrate/HCl, pH 5.5, 75-150 mM NaCl, and 36-44 %(v/v) PEG 200