7B0M
Sugar transaminase from a metagenome collected from troll oil field production water
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 76.405, 76.405, 134.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.370 - 1.430 |
| Rwork | 0.165 |
| R-free | 0.19730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bn1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.019 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.370 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.045 | 2.873 |
| Number of reflections | 84599 | 4170 |
| <I/σ(I)> | 15.9 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 8.3 |
| CC(1/2) | 0.999 | 0.282 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.2 | 291 | 0.2 M Lithium Sulfate,0.1 M Phosphate, 20% w/v PEG 1000 |
