7B0G
TgoT_6G12 binary with 2 hCTPs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2020-06-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.210, 106.770, 231.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.740 - 3.000 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.740 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.204 | 3.171 |
| Number of reflections | 28838 | 2829 |
| <I/σ(I)> | 9.21 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.998 | 0.292 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 20K and 0.1 M MES-NaOH pH6.5 |
