7B0F
TgoT_6G12 Binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-01-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 164.490, 111.920, 74.100 |
Unit cell angles | 90.00, 110.39, 90.00 |
Refinement procedure
Resolution | 46.700 - 2.797 |
R-factor | 0.2315 |
Rwork | 0.231 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tgo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.710 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.700 | 2.897 |
High resolution limit [Å] | 2.797 | 2.797 |
Rmerge | 0.175 | 1.737 |
Number of reflections | 31065 | 3015 |
<I/σ(I)> | 7.18 | |
Completeness [%] | 99.6 | |
Redundancy | 3.5 | |
CC(1/2) | 0.990 | 0.319 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 20K and 0.1 M MES-NaOH pH6.5 |