7B0D
Sugar transaminase from Archaeoglobus veneficus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.227, 105.953, 111.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.287 - 1.270 |
| Rwork | 0.152 |
| R-free | 0.17470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nyu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.665 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.290 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.040 | 1.352 |
| Number of reflections | 212879 | 9242 |
| <I/σ(I)> | 17.3 | 0.9 |
| Completeness [%] | 97.7 | 87 |
| Redundancy | 5 | 3.6 |
| CC(1/2) | 1.000 | 0.305 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.2 | 291 | 0.1 M phosphate/citrate 20 % w/v PEG 6K |
