7B07
TgoT_6G12 apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.890, 110.490, 111.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.530 - 3.099 |
| R-factor | 0.2618 |
| Rwork | 0.262 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.660 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.530 | 3.210 |
| High resolution limit [Å] | 3.099 | 3.099 |
| Rmerge | 0.193 | 2.756 |
| Number of reflections | 132742 | 1621 |
| <I/σ(I)> | 8.72 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.998 | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 20K and 0.1 M MES-NaOH pH 6.5 |
