7AY0

Crystal structure of truncated USP1-UAF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41
Unit cell lengths	119.570, 119.570, 195.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.780 - 3.600
R-factor	0.2366
Rwork	0.235
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5CVN (side chains removed) 5cvl
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.550	3.754
High resolution limit [Å]	3.600	3.602
Rmeas	0.258	1.698
Rpim	0.108	0.727
Number of reflections	25470	1264
<I/σ(I)>	6.4	1.7
Completeness [%]	80.3	34.2
Redundancy	5.7	5.4
CC(1/2)	0.992	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	10% w/v PEG4000, 100 mM MES/Imidazole pH 6.0-6.5, 30 mM CaCl2, 30 mM MgCl2, 20-25% glycerol v/v