7AX7

Crystal structure of the Xyl-CE4 domain of a multidomain xylanase from the hindgut metagenome of Trinervitermes trinervoides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-09
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	40.130, 59.700, 86.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.541 - 2.050
R-factor	0.176291932968
Rwork	0.173
R-free	0.24278
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c71
RMSD bond length	0.006
RMSD bond angle	0.708
Data reduction software	d*TREK (1.12_2829)
Data scaling software	d*TREK (1.12_2829)
Phasing software	PHASER
Refinement software	PHASER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.070	2.120
High resolution limit [Å]	1.930	2.050
Rpim	0.019
Number of reflections	13512	722
<I/σ(I)>	2.74
Completeness [%]	99.0	96.9
Redundancy	2
CC(1/2)	0.999	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292.2	10 mg/mL in 20 mM Tris-HCl pH 7.5, 10 mM NaCl, 8 % (w/v) PEG 4000