7AW9
CCAAT-binding complex and HapX bound to Aspergillus fumigatus cccA DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 76.840, 127.660, 198.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| R-factor | 0.2475 |
| Rwork | 0.245 |
| R-free | 0.29070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7aw7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.053 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.038 | 0.664 |
| Number of reflections | 12478 | 988 |
| <I/σ(I)> | 18.5 | 2.1 |
| Completeness [%] | 97.9 | 99.1 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris-HCl pH 8.5, 25 % PEG4000 |
