7AW7

CCAAT-binding complex and HapX bound to Aspergillus nidulans cccA DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	76.150, 133.150, 199.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.400
R-factor	0.2544
Rwork	0.252
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y37 5vpe
RMSD bond length	0.002
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	3.500
High resolution limit [Å]	3.400	3.400
Rmerge	0.058	0.527
Number of reflections	14326	1166
<I/σ(I)>	14.7	2.8
Completeness [%]	99.6	99.9
Redundancy	5.2	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium fluoride, 20% PEG3350