7AVO
Structure of marine actinobacteria clade rhodopsin (MacR) in orange form in P1211 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97241 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.688, 102.009, 56.601 |
| Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
| Resolution | 48.940 - 1.700 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jsi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.940 | 48.940 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.083 | 0.022 | 1.765 |
| Rmeas | 0.086 | 0.023 | 1.829 |
| Rpim | 0.022 | 0.006 | 0.474 |
| Total number of observations | 5173 | 37119 | |
| Number of reflections | 48957 | 350 | 2557 |
| <I/σ(I)> | 25.5 | 181.8 | 1.7 |
| Completeness [%] | 98.0 | 98.3 | 97.3 |
| Redundancy | 14.5 | 14.8 | 14.5 |
| CC(1/2) | 1.000 | 1.000 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 1.0 M NH4SO4, 0.1 M NaOAc pH 5.2 |
