7AVG
Perdeuterated hen egg-white lysozyme at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.7999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.670, 30.970, 33.740 |
| Unit cell angles | 89.44, 72.82, 67.50 |
Refinement procedure
| Resolution | 32.010 - 1.000 |
| R-factor | 0.121 |
| Rwork | 0.120 |
| R-free | 0.13410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yeo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.616 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.010 | 1.036 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.080 | 0.262 |
| Rmeas | 0.088 | 0.284 |
| Rpim | 0.034 | 0.109 |
| Number of reflections | 49991 | 4880 |
| <I/σ(I)> | 15.07 | 6.92 |
| Completeness [%] | 97.7 | 95.5 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.992 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.5 | 291 | sodium nitrate, sodium acetate pD 4.5 |
