7AVC

DoBi scaffold based on PIH1D1 N-terminal domain

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	H 3 2
Unit cell lengths	72.723, 72.723, 192.775
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.270 - 1.200
R-factor	0.13984
Rwork	0.136
R-free	0.15419
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4psf
RMSD bond length	0.017
RMSD bond angle	1.921
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.270	38.300	1.220
High resolution limit [Å]	1.200	6.580	1.200
Rmerge	0.247	0.207	1.215
Rmeas	0.261	0.218	1.320
Rpim	0.083	0.068	0.500
Number of reflections	59704	438	2225
<I/σ(I)>	5.4	9.7	1.3
Completeness [%]	97.1	99.5	74.4
Redundancy	9.6	9.6	6.4
CC(1/2)	0.978	0.976	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% glycerol, 4M sodium formate