7AV9
Crystal Structure of the second bromodomain of Pleckstrin homology domain interacting protein (PHIP) in space group C2
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.490, 27.020, 55.940 |
| Unit cell angles | 90.00, 100.50, 90.00 |
Refinement procedure
| Resolution | 14.510 - 1.230 |
| Rwork | 0.118 |
| R-free | 0.14600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mb3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.885 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.510 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Number of reflections | 34009 | 1388 |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 96.9 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.997 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG8000, 0.04M potassium phosphate monobasic |
