7AUU
Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1-nose mutant in complex with InsP6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979257 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.120, 62.120, 92.472 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.540 - 2.450 |
R-factor | 0.2081 |
Rwork | 0.206 |
R-free | 0.25540 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7aui |
RMSD bond length | 0.004 |
RMSD bond angle | 1.306 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.540 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.058 | 0.624 |
Rpim | 0.016 | 0.178 |
Number of reflections | 7984 | 873 |
<I/σ(I)> | 21.3 | 3.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.9 | 12.9 |
CC(1/2) | 0.999 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG 3350, 0.1 M Bis-Tris propane pH 6.5, 0.2 M Sodium nitrate. Protein:precipitant ratio 3:1. Protein buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 10 mM InsP6. |