7AUL
Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1 in complex with 5-InsP7 in presence of Mg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979260 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.634, 61.634, 95.978 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.690 - 1.850 |
R-factor | 0.2603 |
Rwork | 0.259 |
R-free | 0.28140 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7auk |
RMSD bond length | 0.005 |
RMSD bond angle | 1.414 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.990 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.078 | 0.650 |
Rpim | 0.021 | 0.183 |
Number of reflections | 18607 | 1124 |
<I/σ(I)> | 17.9 | 3.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.2 | 13.6 |
CC(1/2) | 0.998 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 25% PEG 3350, 0.1 M Sodium acetate pH 4.5, 0.1 M NaCl, 5 mM Magnesium chloride. Protein buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 5 mM 5-InsP7. |