7AUF

anammox-specific acyl carrier protein from Kuenenia stuttgartiensis; normal refinement

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000000
Spacegroup name	P 64
Unit cell lengths	76.810, 76.810, 30.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.400 - 1.760
R-factor	0.2327
Rwork	0.230
R-free	0.28620
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.394
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.400	1.900
High resolution limit [Å]	1.760	1.760
Rpim	0.025	0.652
Number of reflections	13924	1123
<I/σ(I)>	16.4
Completeness [%]	91.6
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	28% (v/v) PEG 400, 200 mM calcium acetate, 100 mM sodium acetate pH 4.5 and 10 mM zinc chloride.