7ASV

Crystal structure of tWHD2 of Rpc5 subunit of human RNA Polymerase III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	P 1 21 1
Unit cell lengths	41.110, 75.760, 62.400
Unit cell angles	90.00, 103.57, 90.00

Refinement procedure

Resolution	30.330 - 1.550
Rwork	0.180
R-free	0.21000
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.147
Data reduction software	xia2 (0.3.8.0)
Data scaling software	xia2 (0.3.8.0)
Phasing software	PHENIX (phenix-1.18.1-3865)
Refinement software	PHENIX (phenix-1.18.1-3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.330	1.520
High resolution limit [Å]	1.480	1.480
Rmerge	0.075	0.735
Number of reflections	59533	3246
<I/σ(I)>	11.8
Completeness [%]	96.7
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	3.2-3.8 M Ammonium Acetate and 100 mM Bis-Tris Propane pH 6.5-7
1	VAPOR DIFFUSION, HANGING DROP		277	3.2-3.8 M Ammonium Acetate and 100 mM Bis-Tris Propane pH 6.5-7