7ASU

Crystal structure of tWHD1 of Rpc5 subunit of human RNA Polymerase III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9198
Spacegroup name	P 61 2 2
Unit cell lengths	56.300, 56.300, 275.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.970 - 2.230
Rwork	0.180
R-free	0.22000
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.841
Data reduction software	xia2 (0.3.8.0)
Data scaling software	xia2 (0.3.8.0)
Phasing software	PHENIX (1.18.1-3865)
Refinement software	PHENIX (1.18.1-3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	2.310
High resolution limit [Å]	2.230	2.230
Rmerge	0.050	0.749
Number of reflections	13725	959
<I/σ(I)>	26.9
Completeness [%]	100.0
Redundancy	27.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	3.2 M NaCl, 100 mM Sodium Acetate pH 4.6 and 10 mM ZnCl2
1	VAPOR DIFFUSION, HANGING DROP		277	3.2 M NaCl, 100 mM Sodium Acetate pH 4.6 and 10 mM ZnCl2